Acquired antibodies in contrary to natural antibodies like Anti A or Anti B found in roughly 3 percent of patients and donators Serum. Most of this acquired antibodies have medical significant, especially because of their potentially hemolytic reaction in HND infants.

Identification of these Antibodies through investigating acquired Antibodies versus RBC is critical for collecting healthy blood. The IGM Antibodies, usually, in laboratory and with direct hemagglutination method in temperature below 30°F are identifiable.  Mostly, IGG Antibodies are identifiable through using Antihuman Globulins in indirect Coombs test in room temperature (37° F). Additionally, Some of IGG Incomplete antibodies, especially Rh System antibodies in Albumin environment directly produce Agglutination. It is proven that at presence of 22% bovine albumin direct hemagglutination in most of blood group antibodies are intensifies.

In fact, Albumin acts as bridge for unusual antibodies in blood in a way that helps to better Agglutination through decreasing Zeta potential in blood cells by increasing the dielectric constant.

Low ionic – strength saline can help to decreasing Zeta potential and better hemagglutination. These saline may contain Glycine or other Nonionic materials that prevent red blood cells Lysis in low ionic power. This saline can contain Albumin too.